Before using the Flow Tool, sequences of CBV and TTM maps need to be created using the MR Analysis Tool. These sequences should contain the same number of images, and be of corresponding slices. These sequences should ideally contain at least 5 contiguous slices, a minumum of three is required.
To use the Regional histogram functionality, the Coreg Tool needs to be open, and either the co-registration of the CBV/TTM maps to the Talairach atlas brain needs to be performed, or the tair file containing the registration parameters should be opened in the Coreg Tool. Also, the Talairach Tool, specifying the location of the Talairach atlas database files needs to be open.